Writer: Bill Finger
Cover Artist: Bob Kane
An advertisement appears on the newspapers about a man under the alias of A. Rekoj, summoning practical jokers for some kind of job. The applicants perform in front of the old man Rekoj and after a long casting process, only the most reckless are chosen. Rekoj tells them to pick up some objects and then reveals his true identity as Joker. The criminal has framed the various jokesters by making them touch weapons used in crimes and with the upper hand, he tells his new henchmen that they are going to create havoc in the city by playing some of the most ruthless practical jokes. In this way, the city becomes chaotic and the police are baffled with these new crimes.

Batman and Robin investigate this cases and they are informed about the Joker's next target. The duo go to the place to prevent Joker from stealing a valuable gem, but they are confronted by dozens of Jokers. Outnumbered, the dynamic duo are defeated and when they recover, they learn that while they were fighting fake Jokers, the real Joker was stealing a true gem elsewhere. Joker calls Batman and gives him another clue for his next hit, but Batman can't solve the riddle.

The answer comes to Bruce later as he learns that a famous Duke is arriving soon at the city. Batman deduces that Joker is going to attack the duke and his deduction proves right when the Joker in fact kidnaps the duke and disguises as him in order to steal ten thousand dollars. When Batman and Robin arrive to the place and try to stop him, they have to fight all of Joker's practical jokers and the clown prince of crime manages to escape. After defeating all of Joker's henchmen, Batman and Robin follow him to the railroad station, where Joker takes a train out of the city. Batman and Robin also get on the train and they chase Joker across the wagons all the way to the top, where during a final battle, Joker falls to the river below.

Batman and Robin recover the stolen money and there is no sign of the Joker.